Rat Antigens
Slides and images prepared by Dr. Jerrold M. Ward, Larry Faucette (both of NIAID, NIH) and Christine Dijkstra (VU University Medical Center)
CD3: Lymph Node, Spleen, Thymus
ED-1: Lymph Node, Spleen, Thymus
ED-2: Lymph Node, Spleen, Thymus
ED-3: Spleen
IgD: Spleen
IgM: Lymph Node, Spleen
MHC II: Lymph Node, Peyer's Patch, Spleen
Ox-8: Liver, Lung, Lymph Node, Peyer's Patch, Small Intestine, Spleen, Thymus
Rat Kappa Light Chains: Spleen
W3-13: Lymph Node, Spleen
W3-25: Lymph Node, Peyer's Patch, Spleen
Detection of Rat Hematopoietic Antigens in Paraffin and Frozen Sections
Antibody |
Major Cells Expressing Antigen |
Source |
Product
Number |
Clone |
| CD3 |
All T cells |
Dako |
A0452 |
|
| CD4 |
T-helper cells |
Serotec |
MCA55G |
W3/25 |
| CD5 |
Subset of T lymphocytes, most thymocytes and NK cells |
Serotec |
MCA52G |
OX-19 |
| CD8 |
Subset of T lymphocytes, most thymocytes and NK cells |
Serotec |
MCA48G |
OX-8 |
| CD11b* |
Most macrophages, Kupffer cells, also dendritic cells, granulocytes |
Serotec |
MCA275GA |
OX-42 |
| CD25 |
Activated rat T cells |
Serotec |
MCA273R |
OX-39 |
| CD43 |
All leucocytes with the exception of B lymphocytes |
Serotec |
MCA54G |
W3/13 |
| CD45RA |
B cells among thoracic duct lymphocytes with little labeling in bone marrow and none on thymocytes |
Serotec |
MCA340GA |
OX-33 |
| CD45RC |
B cells |
Serotec |
MCA53R |
OX-22 |
| CD68 |
Myeloid cells |
Serotec |
MCA341R |
ED1 |
| CD163 |
50% peritoneal macrophages, splenic & most other macrophages |
Serotec |
MCA342GA |
ED2 |
| CD169* |
Macrophages |
Serotec |
MCA343R |
ED3 |
| IgM |
B cells |
Vector |
BA-2020 |
|
| MHC Class II |
B cells |
Serotec |
MCA46G |
OX-6 |
| Immunoglobulin human kappa light chains |
Immunoglobulin-producing B cells |
Dako |
A0191 |
|
*works only with frozen sections
Slides and images were prepared by Dr. Jerrold M. Ward, Larry Faucette, Cindy Erexson (all from the Comparative Medicine Branch, NIAID, NIH) and Dr. Georgio Cattoretti (formerly of Columbia University).
Adapted from Ward JM, Erexson CR, Faucette LJ, Foley JF, Dijkstra C, Cattoretti G. Immunohistochemical markers for the rodent immune system. Toxicol Pathol. 2006;34(5):616-30.
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