Cell Surface Antigens

Mouse spleen showing double stain for CD3 (T cells, red) and B220 (B cells, brown), Bouin's fixation

Ras p21 on cell membrane of BALB/c 3T3 cells transfected by H-ras and injected into mice causing a sarcoma. Note that only a small proportion of tumor cells are immunoreactive. Bouin's fixative must be used. Some investigators have shown apparent immunoreactivity in the cytoplasm only with formalin fixation.For some of these, these results may represent nonspecific staining. Proper controls can help differentiate between specific and nonspecific staining.

Ras p21 on Harvey sarcoma virus-infected cells. Bouin's fixative.

Ras p21 on rat bladder tumor cells, Bouin's fixative.

CD3 (rabbit polyclonal) on Bouin's fixed mouse spleen. CD3 is found in many species and paraffin sections can be used with fixatives other than formalin.

B220 in B cell zones of mouse spleen, paraformaldehyde fixation, biotinylated mouse monoclonal antibody.This antibody will also work with formalin fixed tissues after microwave pretreatment.

OX-8 (mouse monoclonal) on cell surface of large granular leukemic lymphocytes in rat spleen, Bouin's fixative. This antibody will also immunoreact with formalin fixed tissues after microwave pretreatment.

Retroviral antigens (membrane and cytoplasmic) - can be easily demonstrated in formalin-fixed tissues sections of animals and humans.

HIV p24 in cytoplasm and on cell membranes of syncytial and other cells in vitro, formalin fixation.

SIV antigens in giant cell in lamina propria of colon of monkey, polyclonal antibody.

SIV antigens (polyclonal antibody) in brain lesion composed primarily of macrophages.

HIV p24 in blood monocyte in HIV infection. Formalin fixation. (Courtesy of Dr. Femi Williams).

Rauscher leukemia virus p30 in megakaryocytes of mouse spleen. Formalin.

Transforming growth factor-alpha on cell surface of hepatocytes in prenoeplastic focus (polyclonal antibody to pro-form).

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