Mouse Antigens
Slides and images were prepared by Dr. Jerrold M. Ward, Larry Faucette, Cindy Erexson (all from the Comparative Medicine Branch, NIAID, NIH) and Dr. Georgio Cattoretti (formerly of Columbia University).
Bcl-2: Spleen
Bcl-6: Spleen
Caspase 3: Thymus
CD3: Lymph Node, Spleen, Thymus
CD21/Oct 2: Spleen
CD23/Oct 8: Spleen
CD40: Lymph Node, Spleen
CD45R: Lymph Node, Peyer's Patch, Spleen
CD45R/Bcl-6: Spleen
F480: Liver, Spleen
Human Kappa Light Chains: Spleen
IRF4/Bcl-6: Peyer's Patch, Spleen
MPO: Spleen
Pax-5: Spleen
Tdt: Thymus
Detection of Mouse Hematopoietic Antigens in Paraffin Sections of Thymus, Spleen and Lymph Nodes
| Antibody |
Major Cells
Expressing Antigen |
Source |
Product Number
or Clone
|
Bcl-2
|
Germinal center B cells |
Santa Cruz Biotechnology |
sc-7382 |
| Bcl-6 |
Germinal center B cells |
Santa Cruz Biotechnology |
sc-858
|
| BclX |
Bcl-XS and Bcl-XL proteins |
Lab Vision |
2H12 (Ab-1)
|
Caspase-3 Cleaved
|
Apoptotic cells |
Cell Signaling
Technology |
9664
|
| CD3 |
Pan T lymphocytes |
Dako |
A0452 |
CD19
|
B cells |
Cell Signaling Technology |
3574 |
| CD21 |
B cells
Dendritic cells |
Santa Cruz Biotechnology |
sc-7028FITC
|
| CD23 |
Monocytes, Follicular B cells |
Santa Cruz Biotechnology |
sc-7024 |
| CD30 |
Activated T and B cells |
BD Biosciences |
553824 |
| CD43 |
T and B cells
Myeloid cells |
BD Biosciences |
552366 |
| CD45R/B220 |
B cells |
BD Biosciences |
553850
|
CD138
(Syndecan-1)
|
B cells |
BD Biosciences |
553714 |
| F4/80 |
Histiocytes
Macrophages |
Serotec |
MCA497BB
|
| Immunoglobulin human kappa light chains |
Immunoglobulin-producing B cells |
Dako |
A0191
|
IRF4
|
B cells |
Santa Cruz Biotechnology |
sc-6059
|
| Mac-2 |
Histiocytes
Macrophages |
Cedarlane
Laboratories |
CL8942AP
|
| Myeloperoxidase |
Myeloid cells |
Dako |
A0398 |
NOS2
(Nitric Oxide Synthase) |
Myeloid cells |
Santa Cruz
Biotechnology |
sc-651 |
Pax5
|
Various B cells |
Santa Cruz Biotechnology |
sc1974 |
| PNA-biotin |
B cells, Follicles
Germinal centers |
Vector Laboratories |
B-1075 |
| PU.1 |
B cells, early T cells, and others |
Santa Cruz Biotechnology |
sc-5949 |
| TdT |
T cells |
Lab Vision |
Ab-2 |
*works only with frozen sections
Slides and images were prepared by Dr. Jerrold M. Ward, Larry Faucette, Cindy Erexson (all from the Comparative Medicine Branch, NIAID, NIH) and Dr. Georgio Cattoretti (formerly of Columbia University).
Adapted from Ward JM, Erexson CR, Faucette LJ, Foley JF, Dijkstra C, Cattoretti G. Immunohistochemical markers for the rodent immune system. Toxicol Pathol. 2006;34(5):616-30.
back to top