Research Technologies Branch

Protocols

Culture Dishes for Use on the Confocal Microscopes

Plastic Slides With Chambers

Good Ones

• Lab-Tek II chamber slides, Nunc number 154461
  Order from Fisher Scientific, number 12-565-6 (2 chamber)

Not Good

• Gasket material left behind
• Lab-Tek chamber slide, Nunc number 177429
  Available from Fisher Scientific, number 12-565-20 (2 chamber)

Glass Slides With Chambers

Same as above, but the slide is glass rather than plastic.
Lab-Tek chamber slide, Nunc number 177402 (2 chamber)
Available from Fisher Scientific

Chambers With Coverslip Bottoms

• Nunc chambered cover glass
• 1 well 12-565-472
• 2 well 12-565-471
• 4 well 12-565-401 (old # 12-565-103n)
• 8 well 12-565-470
• Order from Fisher Scientific (on Ambis)
  1-800-766-7000
  www.Fishersci.com* (Nongovernment Web site)

96 Well Plates With Coverslip Bottoms

• SO (special optics) 96 well plates, Corning-Costar
  Catalog number: 3614 TC treated; 3615 not treated
  Info at www.scienceproducts.corning.com* (Nongovernment Web site)
  1-800-492-1110
• Whatman Uniview, number 7706-1365
  from Whatman, Clifton, NJ
  www.whatman.com* (Nongovernment Web site)
  1-800-434-7659

Culture Dishes With Coverslip Bottoms

Small coverslip in center of bottom, Mat tek number P35G-0-10-C: Mat tek Co, 1-508-881-6771

Entire Dish Bottom Coverslip

Available from Bioptechs, Butler, PA
www.Bioptechs.com* (Nongovernment Web site)
1-877-5483-2355

Note: Plastic slides cannot be used if collection of DIC images is required.

*Some of the links on this page connect to information sources outside of the NIAID and are provided as a convenience for World Wide Web users. Please see the NIAID disclaimer.

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