Technology
Image Capture
Microscopes:
Leica DMIRBE, Leica DMIRB, Zeiss Axiovert 100
Cameras:
Cooke Sensicam QE, Hamamatsu Cameras, Zeiss Axiocam Color
Software:
Image-Pro Plus, Zeiss Axiovision
Image capture includes the use of standard film cameras, high-resolution CCD cameras (either color or monochrome), or video rate CCD cameras for true real time image collection. Images can be collected on either of the Leica inverted microscopes or on the MZ 9.5 dissection microscope. If special microscope configurations are required, please note this when bookings are made.
On microscopes other than the confocal systems, a variety of image capture cameras are available. Traditional film cameras are available for production of 35mm slides if desired. More commonly, images are collected on CCD cameras. The facility has a wide range of cameras. For high-resolution images the Sensicam QE is appropriate, collecting images of 1376 x 1040 pixels. This camera is very sensitive in monochrome mode and is recommended for images of fluorescent labeled samples. A CRI microcolor filter can be put into the beam path to allow for full color images at this resolution. This filter is not recommended for fluorescent samples, but it is very good for brightfield images. For very low light applications (dim fluorescence), an intensified CCD camera is used. For live cell imaging, where very high frame rates are required, a Hamamatsu video camera (30fps monochrome) can be used.
The Leica DMIRBE microscope can be configured in many different ways to accommodate a range of experiments. An x-y joystick stage can be attached for precise movement during imaging. Eppendorf micromanipulators can be attached for microdissection or micromanipulation. Combined with the Eppendorf InjectMan and TransferMan the micromanipulators can be used for microinjection experiments.
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