Research Technologies Branch

Instrument Specifications

• Two lasers: argon, argon-dye
• Multi-parameter analysis and sterile sorting based on up to eight parameters (FS, SS, and six colors)
• Turbo-Sort for high-speed sorting
• OmniComp for interlaser compensation (i.e., PE-Cy5 and APC, PE and TR or PE-TR and TR)

• Two lasers: argon and HeNe
• Multi-parameter analysis and sterile sorting based on up to eight parameters (FS, SS, and six colors)
• Turbo-Sort for high-speed sorting
• Digital Electronics (DiVa option) allowing all detector compensation and 4-way sorting

• 3 lasers: 488nm, 405nm and 633nm solid state
• Multi-parameter analysis and sterile sorting based on up to 15 parameters (FS, SS, 13 colors)
• All Digital acquisition allowing all parameter compensation and four- way sorting
• Single cell cloning into 96 or 24 well plates high-speed sorting (70 psi)

• User-operated benchtop flow cytometer, 12 parameters (10 fluorescent colors and 2 light scatters)
• 4 lasers: 488nm argon, 633nm HeNe, 407nm solid state, and 325nm HeCad with fixed alignment
• Dyes capable of measuring: 488 laser dyes: FITC, PE, PE-Cy5, PE-Cy7, 325 laser dyes: Indo-1, Hoechst, DAPI 633 nm laser dyes: APC or Cy5 and APC-Cy7, 407nm laser dyes: Cascade Blue, Cascade Yellow, Alexa 430, CFP
• User changeable filters, Quartz cuvette flow cell, Pulse processing on all parameters, All color compensation; DiVa digital acquisition software on Win2K PC

• One laser: argon
• Standard five-parameter analysis/double discrimination for DNA analysis
• Macintosh platform (CellQuest) for data acquisition and analysis

• Two lasers: argon, red diode (633nm)
• Six parameter analysis and sorting/doublet discrimination for DNA analysis
• Four-color analysis using FITC, PE, PE-Cy5 (or PerCP), and APC
• Macintosh platform (CellQuest) for data acquisition and analysis

• Capable of measuring up to 100 different analytes/tube.
• Technology: molecular reactions take place on the surface of microspheres.
• Thousands of molecules are attached to the surface of internally color-coded microspheres.
• Assigned color-code identifies the reaction throughout the test
• Magnitude of the biomolecular reaction is measured using a second molecule called a reporter.
• Reporter molecule signals the extent of the reaction by attaching to the molecules on the microspheres.
• Two sources of color: the color-code inside the microsphere and the reporter color on the surface of the microsphere.

• Three lasers: 488nm, 405nm and 633nm solid state
• Multi-parameter analysis and sterile sorting based on up to 12 parameters (FS, SS, 10 colors)
• All Digital acquisition allowing all parameter compensation and four-way sorting
• Single cell cloning into 96 or 24 well plates high-speed sorting (70 psi)

• Two lasers: argon, red diode (633nm)
• Six-parameter analysis and sorting/doublet discrimination for DNA analysis
• Four-color analysis using FITC, PE, PE-Cy5 (or PerCP), and APC
• Macintosh platform (CellQuest) for data acquisition and analysis