Aedes aegypti Annotation 1.0 Released

The National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIAID/NIH) has funded the sequencing, assembly, and annotation of the Aedes aegypti genome through its Microbial Sequencing Centers: the J. Craig Venter Institute (JCVI) and the Broad Institute, Massachusetts Institute of Technology, as well as VectorBase, a NIAID Bioinformatics Resource Center.

A completed computational annotation of the Aedes aegypti 7.6X assembly is now available in GenBank (AAGE00000000) and at VectorBase. Functional assignments and gene accessions are also available. Long-term curation of the sequence and subsequent annotation updates will be the responsibility of VectorBase.

Aedes aegypti Assemblies

Two sequence assemblies of the Aedes genome have been released: a 5-fold (AAGE01000000) and 7.6-fold (AAGE02000000).

7.6-Fold Assembly Statistics

• Total number of sequence reads: 14.9 million
• Total number of sequence reads placed in the assembly: 12.3 million
• Average contig coverage: 7.6X (6.71X in Q20 bases)
• Number of contigs: 36,206
• Total length of combined contigs 1.31 Gb
• Number of scaffolds: 4,758
• Total length of combined scaffolds (including gaps): 1.383 Gb

Supplementary Data Release

Complementary to the sequencing and annotation project described above, NIAID/NIH has also funded a project to provide additional Aedes aegypti genome-related resources. As part of this project, JCVI has released in GenBank 207,079 Aedes aegypti EST sequences and 117,937 BAC end sequences generated from a variety of libraries. The EST accession ranges to date are DV230611-DV235882, DV235883-DV270715, DV300289-DV348598, DV348687-DV389983, DV389984 and DV437762. The BAC end sequence accession ranges are CC065891-CC144307, CC149837-CC155974, and CC841856-CC875159.

Additional information about the Aedes aegypti sequencing project and assembly can be found at the Broad Institute and at JCVI.

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